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dermal fibroblast cell line bj 5ta  (ATCC)


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    Structured Review

    ATCC dermal fibroblast cell line bj 5ta
    (A) BJ-Fucci fibroblasts. <t>BJ-5ta</t> fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.
    Dermal Fibroblast Cell Line Bj 5ta, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 483 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dermal+fibroblast+cell+line+bj+5ta/BJ-5ta/bio_rxiv__2025__09__12__675830-68-3-16
    Average 96 stars, based on 483 article reviews
    dermal fibroblast cell line bj 5ta - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts"

    Article Title: Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts

    Journal: bioRxiv

    doi: 10.1101/2025.09.12.675830

    (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.
    Figure Legend Snippet: (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.

    Techniques Used: Transduction, Fluorescence, Imaging, RNA Sequencing, Expressing

    Related Articles

    Transduction:

    Article Title: Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts
    Article Snippet: The hTERT-immortalized human dermal fibroblast cell line BJ-5ta (source: foreskin of male neonate) was purchased from ATCC (ATCC catalog # CRL-4001).. BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].

    Fluorescence:

    Article Title: Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts
    Article Snippet: The hTERT-immortalized human dermal fibroblast cell line BJ-5ta (source: foreskin of male neonate) was purchased from ATCC (ATCC catalog # CRL-4001).. BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].

    Imaging:

    Article Title: Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts
    Article Snippet: The hTERT-immortalized human dermal fibroblast cell line BJ-5ta (source: foreskin of male neonate) was purchased from ATCC (ATCC catalog # CRL-4001).. BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].

    RNA Sequencing:

    Article Title: Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts
    Article Snippet: The hTERT-immortalized human dermal fibroblast cell line BJ-5ta (source: foreskin of male neonate) was purchased from ATCC (ATCC catalog # CRL-4001).. BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].

    Expressing:

    Article Title: Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts
    Article Snippet: The hTERT-immortalized human dermal fibroblast cell line BJ-5ta (source: foreskin of male neonate) was purchased from ATCC (ATCC catalog # CRL-4001).. BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].BJ-5ta retained normal fibroblast growth phenotypes and did not exhibit transformed phenotypes, as described in the original publication [ ].



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    dermal fibroblast cell line bj 5ta  (ATCC)
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    ATCC dermal fibroblast cell line bj 5ta
    (A) BJ-Fucci fibroblasts. <t>BJ-5ta</t> fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.
    Dermal Fibroblast Cell Line Bj 5ta, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dermal+fibroblast+cell+line+bj+5ta/BJ-5ta/bio_rxiv__2025__09__12__675830-68-3-16
    Average 96 stars, based on 1 article reviews
    dermal fibroblast cell line bj 5ta - by Bioz Stars, 2026-10
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    		  Buy from Supplier
    human dermal fibroblasts bj 5ta cell line  (ATCC)
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    ATCC human dermal fibroblasts bj 5ta cell line
    (A) Representative western blot probing PIEZO1 and α-ACTININ from various cell lines. For protein lanes: 1, sonicated HCF; 2, HCF; 3, 95 °C boiled HCF; 4, HUVEC; 5, PAOEC; 6, HCF (high passage number); 7, <t>BJ-5ta;</t> 8, HEK293T; 9, MCF-7; 10, HeLa; 11, LnCaP; 12, HEK293T- Piezo1-/- . (B) Example of the quantified intensity level of PIEZO1 signals and α-ACTININ signals from multiple cell lines.
    Human Dermal Fibroblasts Bj 5ta Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human dermal fibroblast cell line bj 5ta  (ATCC)
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    ATCC human dermal fibroblast cell line bj 5ta
    (A) Representative western blot probing PIEZO1 and α-ACTININ from various cell lines. For protein lanes: 1, sonicated HCF; 2, HCF; 3, 95 °C boiled HCF; 4, HUVEC; 5, PAOEC; 6, HCF (high passage number); 7, <t>BJ-5ta;</t> 8, HEK293T; 9, MCF-7; 10, HeLa; 11, LnCaP; 12, HEK293T- Piezo1-/- . (B) Example of the quantified intensity level of PIEZO1 signals and α-ACTININ signals from multiple cell lines.
    Human Dermal Fibroblast Cell Line Bj 5ta, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.

    Journal: bioRxiv

    Article Title: Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts

    doi: 10.1101/2025.09.12.675830

    Figure Lengend Snippet: (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.

    Article Snippet: The hTERT-immortalized human dermal fibroblast cell line BJ-5ta (source: foreskin of male neonate) was purchased from ATCC (ATCC catalog # CRL-4001).

    Techniques: Transduction, Fluorescence, Imaging, RNA Sequencing, Expressing

    (A) Representative western blot probing PIEZO1 and α-ACTININ from various cell lines. For protein lanes: 1, sonicated HCF; 2, HCF; 3, 95 °C boiled HCF; 4, HUVEC; 5, PAOEC; 6, HCF (high passage number); 7, BJ-5ta; 8, HEK293T; 9, MCF-7; 10, HeLa; 11, LnCaP; 12, HEK293T- Piezo1-/- . (B) Example of the quantified intensity level of PIEZO1 signals and α-ACTININ signals from multiple cell lines.

    Journal: Bio-protocol

    Article Title: Western Blotting and Immunoprecipitation of Native Human PIEZO1 Channels

    doi: 10.21769/BioProtoc.5385

    Figure Lengend Snippet: (A) Representative western blot probing PIEZO1 and α-ACTININ from various cell lines. For protein lanes: 1, sonicated HCF; 2, HCF; 3, 95 °C boiled HCF; 4, HUVEC; 5, PAOEC; 6, HCF (high passage number); 7, BJ-5ta; 8, HEK293T; 9, MCF-7; 10, HeLa; 11, LnCaP; 12, HEK293T- Piezo1-/- . (B) Example of the quantified intensity level of PIEZO1 signals and α-ACTININ signals from multiple cell lines.

    Article Snippet: Human dermal fibroblasts BJ-5ta cell line (ATCC, catalog number: CRL-4001, hTERT-immortalized human foreskin fibroblast line, derived from a neonatal male) 7.

    Techniques: Western Blot, Sonication